Product Description
General PAF(Platelet Activating Factor) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: PAF-Acether; AGEPC; Acetyl-Glyceryl-Ether-Phosphorylcholine
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 14.4 pg/mL
Standard: 3000 pg/mL
Detection Range: 46.88-3000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Metabolic pathway;Infection immunity;Cardiovascular biology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Platelet Activating Factor(PAF) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Activating Factor(PAF). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Activating Factor(PAF) in the samples is then determined by comparing the OD of the samples to the standard curve.
