Product Description
General LXA4(Lipoxin A4) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: LX-A4
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 175.4 pg/mL
Standard: 40000 pg/mL
Detection Range: 625-40000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Signal transduction;Metabolic pathway;Infection immunity;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Lipoxin A4(LXA4) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipoxin A4(LXA4). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipoxin A4(LXA4) in the samples is then determined by comparing the OD of the samples to the standard curve.
