Product Description
General IAA(Indole 3 Acetic Acid) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.89 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: tissue or cell culture supernates
Assay Length: 2h
Research AreaResearch Area: Signal transduction;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Indole 3 Acetic Acid(IAA) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Indole 3 Acetic Acid(IAA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Indole 3 Acetic Acid(IAA) in the samples is then determined by comparing the OD of the samples to the standard curve.
