Product Description
General GA(Gibberellic Acid) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: GA3; Gibberellin A3; Gibberellins
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 52.1 ng/mL
Standard: 10000 ng/mL
Detection Range: 156.25-10000 ng/mL
Sample Type: tissue or cell culture supernates
Assay Length: 2h
Research AreaResearch Area: Nutrition metabolism;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gibberellic Acid(GA) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gibberellic Acid(GA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gibberellic Acid(GA) in the samples is then determined by comparing the OD of the samples to the standard curve.
