Product Description
General cGMP(Cyclic Guanosine Monophosphate) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: c-GMP; 3',5'-Cyclic GMP; Cyclic 3',5'-GMP; Guanosine 3',5'-Cyclic Phosphate
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 45.2 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Signal transduction;Metabolic pathway;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cyclic Guanosine Monophosphate(cGMP) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclic Guanosine Monophosphate(cGMP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclic Guanosine Monophosphate(cGMP) in the samples is then determined by comparing the OD of the samples to the standard curve.
