Product Description
General 5-HIAA(5-Hydroxyindoleacetic Acid) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: 5HIAA
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 1.93 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample Type: serum, plasma, urine, cerebrospinal fluid and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Metabolic pathway;Tumor immunity;Endocrinology;Urology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxyindoleacetic Acid(5-HIAA) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5-Hydroxyindoleacetic Acid(5-HIAA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxyindoleacetic Acid(5-HIAA) in the samples is then determined by comparing the OD of the samples to the standard curve.
