GAPDH monoclonal antibody

Product Description

GAPDH monoclonal antibody is available at gentaur for next week delivery

Background: GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Applications:  WB ICC

Purification&Purity: ProL affinity purified

Storage&Stability:  Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity:  GAPDH monoclonal antibody detects endogenous levels of GAPDH protein.

W4BiowMW:  36 kDa

Reactivity: Human,Mouse,Rat

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

peptide

Alternative Name:

GAPDH, G3P, 38 kDa BFA-dependent ADP-ribosylation substrate, aging associated gene 9 protein, Aging-associated gene 9 protein, BARS-38, cb609, EC 1.2.1.12, Epididymis secretory sperm binding protein Li 162eP, G3P, G3PD, G3PDH, GAPD, GAPDH, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL-S-162eP, KNC-NDS6, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38 component, OCT1 coactivator in S phase, 38-KD component, peptidyl cysteine S nitrosylase GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, wu:fb33a10,

Western blot analysis:  Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/5000 dilution. Positive control: Lane 1: HepG2 Lane 2: Hela Lane 3: PC12 Lane 4: NIH/3T3 Lane 5: MCF-7 Lane 6: Rabbit liver Lane 7:Zebrafish

Immunohistochemistry: ICC staining GAPDH in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunofluorescence analysis:

Host:  Mouse

Swiss-Prot: P04406

 

 

 

 

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