Product Description
Flotillin-2 (R144) polyclonal antibody is available at gentaur for next week delivery
Background: Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry a evolutionarily conserved domain called the prohibitin homology domain (PHB). Flotillin members are ubiquitously expressed and located to non-caveolar microdomains (lipid rafts) on the cell plasma membrane where they support signal transduction and regulate lipid raft motility and localization. Two flotillin members have been described, flotillin-1 and flotillin-2. In addition to its colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found at compartments of the endocytic and autophagosomal pathways, such as recycling/late endosomes, the Golgi complex, as well as the nucleus. Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. However, overexpressed flotillin-2 has also been found in the late endosome. Both flotillin-1 and flotillin-2 are commonly used as lipid raft associated markers.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Flotillin-2 (R144) polyclonal antibody detects endogenous levels of Flotillin-2 protein.
W4BiowMW: ~ 49 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1.Alternative Name:
Flotillin-2; Epidermal surface antigen; ESA; Membrane component chromosome 17 surface marker 1; ESA1; M17S1; FLOT2Western blot analysis: Western blot (WB) analysis of FLOT2 pAb at 1:500 dilution Lane1:A375 whole cell lysate(40ug) Lane2:SCC-7 whole cell lysate(40ug) Lane3:The Brain tissue lysate of Mouse(40ug) Lane4:C6 whole cell lysate(40ug)
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: Q14254
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