ERK1/2 (T202/Y204) polyclonal antibody

Product Description

ERK1/2 (T202/Y204) polyclonal antibody is available at gentaur for next week delivery

Background: The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos-phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.

Applications:  WB

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  ERK1/2 (T202/Y204) polyclonal antibody detects endogenous levels of ERK1/2 protein.

W4BiowMW:  ~ 45 kDa

Reactivity: Human,Rat

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic peptide, corresponding to amino acids 180-220 of Human ERK1.

Alternative Name:

Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1; MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK1; ERK2; PRKM1; PRKM2

Western blot analysis:  Western blot (WB) analysis of ERK1/2 pAb at 1:500 dilution Lane1:U-87MG whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(40ug) Lane3:EC9706 whole cell lysate(40ug)

Immunohistochemistry: Western blot (WB) analysis of ERK1/2 (T202/Y204) pAb at 1:500 dillution Lane1:Hela whole cell lysate(20μg) Lane2:NIH-3T3 whole cell lysate(40μg) Lane3:H9C2 whole cell lysate(40μg)

Immunofluorescence analysis:

Host:  Rabbit

Swiss-Prot: P27361/P28482

 

 

 

 

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