Product Description
eNOS (Phospho-S615) polyclonal antibody is available at gentaur for next week delivery
Background: NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: eNOS (Phospho-S615) polyclonal antibody detects endogenous levels of eNOS protein only when phosphorylated at Ser615.
W4BiowMW: ~ 140 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Serine 615.Alternative Name:
Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII; NOS3Western blot analysis: Western blot (WB) analysis of eNOS (Phospho-S615) polyclonal antibody at 1:500 dilution LaneA:K562 whole cell lysate LaneB:The Spleen tissue lysate of Mouse LaneC:The Spleen tissue lysate of Rat
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P29474
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