Product Description
eIF4E (T203) polyclonal antibody is available at gentaur for next week delivery
Background: eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.
Applications: WB IHC IP
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: eIF4E (T203) polyclonal antibody detects endogenous levels of eIF4E protein.
W4BiowMW: ~ 25 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to amino acids 171-210 of Human eIF4E.Alternative Name:
eIF4E ( phospho S209 ); eIF-4E; eIF4E; mRNA cap-binding protein; eIF-4F 25 kDa subunit; EIF4E; 1977; EIF4E; 133440; P06730; CBP; EIF4EL1; EIF4F; MGC111573;Western blot analysis: Western blot (WB) analysis of eIF4E (T203) pAb at 1:1000 dilution Lane1:Hela whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:The Spleen tissue lysate of Rat(40ug) Lane4:3T3-L1 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)
Immunohistochemistry: Immunohistochemistry (IHC) analyzes of eIF4E (T203) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Immunofluorescence analysis: Immunoprecipitation of HEK293T cell lysate using eIF4E (T203) polyclonal antibody (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3).Lane 1 is 30% input.The western blot was probed using eIF4E (T203) #BS3432.“↑”（supernatant）;“↓(deposition）
Host: Rabbit
Swiss-Prot: P06730
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