Product Description
eIF4E polyclonal antibody is available at gentaur for next week delivery
Background: The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA “cap” during an early step in the initiation of protein synthesis . eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A, and the eIF3 complex.
Applications: WB IHC IF IP
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: EIF4E polyclonal antibody detects endogenous levels of EIF4E protein.
W4BiowMW: ~ 25 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant full length Human eIF4E.Alternative Name:
eIF4E ( phospho S209 ); eIF-4E; eIF4E; mRNA cap-binding protein; eIF-4F 25 kDa subunit; EIF4E; 1977; EIF4E; 133440; P06730; CBP; EIF4EL1; EIF4F; MGC111573;Western blot analysis: Western blot analysis of eIF4E polyclonal antibody
Immunohistochemistry: Immunohistochemistry (IHC) analysis of eIF4E polyclonal antibody in paraffin-embedded Human Kidney tissue.
Immunofluorescence analysis: Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug EIF4E antibody.
Host: Rabbit
Swiss-Prot: P06730
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