Product Description
eEF2K polyclonal antibody is available at gentaur for next week delivery
Background: eEF2K is 105 kDa protein that is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38γ, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insulin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this results in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations.
Applications: WB IF
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: eEF2K polyclonal antibody detects endogenous levels of eEF2K protein.
W4BiowMW: ~ 110 kDa
Reactivity: Human,Mouse
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant full length Human eEF2K.Alternative Name:
Elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; EEF2KWestern blot analysis: Western blot analysis of extracts of various cell lines, using EEF2K antibody.
Immunohistochemistry: Immunofluorescence analysis of MCF-7 cell using EEF2K antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: O00418
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