Product Description
DNA Ligase I polyclonal antibody is available at gentaur for next week delivery
Background: Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I. A combination of DNA polymerase epsilon, PCNA, replication factor C, replication protein A, and DNA ligase I is well-suited to the task of creating nucleotide excision repair patches.
Applications: WB IHC IF
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: DNA Ligase I polyclonal antibody detects endogenous levels of DNA Ligase I protein.
W4BiowMW: ~ 140 kDa
Reactivity: Human,Mouse
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant full length Human DNA Ligase I.Alternative Name:
DNA ligase 1; DNA ligase I; DNLI1; LIG 1; lig1; Ligase I DNA ATP dependent; Polydeoxyribonucleotide synthase [ATP] 1;Western blot analysis: Western blot analysis of extracts of various cell lines, using DNA Ligase I antibody.
Immunohistochemistry: Immunohistochemistry (IHC) analysis of DNA Ligase I polyclonal antibody in paraffin-embedded Human Breast Cancer tissue.
Immunofluorescence analysis: Immunofluorescence analysis of MCF7 cells, using DNA Ligase I polyclonal antibody
Host: Rabbit
Swiss-Prot: P18858
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