Product Description
DDDDK-tag polyclonal antibody is available at gentaur for next week delivery
Background: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
Applications: WB IF
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: DDDDK-tag polyclonal antibody detects over-expressed or recombinant proteins containing the DDDDK epitope tag.
W4BiowMW: N/A
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide DDDDK-tagAlternative Name:
DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag,ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; DDDDKWestern blot analysis: Western blot (WB) analysis of DDDDK-tag pAb at 1:5000 dillution Lane1:HEK293T whole cell lysate Lane2:HEK293T whole cell lysate,transfected with pcDNA3.1-DDDDK-p62-HA.
Immunohistochemistry: IF image of AP0007 stained HEK293T cells, transfected with pcDNA3.1-HA-DDDDK-p62 #PPL00549-2b. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody DDDDK-tag pAb #AP0007(1:200) and the antibody HA-tag (4G3) mAb #AP0005M(1:200) at 5µg/ml overnight at +4°C. The secondary antibody (Green) wasGoat anti-Rabbit IgG (H+L) -FITC#BS10950 and the secondary antibody (Red) was Goat anti-Mouse IgG (H+L)-TRITC#BS11502. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: N/A
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