Product Description
Cytochrome c polyclonal antibody is available at gentaur for next week delivery
Background: Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form. This event further triggers caspase-3 activation and eventually leads to apoptosis.
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Cytochrome c polyclonal antibody detects endogenous levels of Cytochrome c protein.
W4BiowMW: ~ 17 kDa
Reactivity: Human,Rat,Mouse
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant protein of human Cytochrome c.Alternative Name:
Cytochrome c; CYCS; CYCWestern blot analysis: Western blot (WB) analysis of Cytochrome c polyclonal antibody at 1:2000 dilution Lane1:The Heart tissue lysate of Mouse(40ug) Lane2:The Muscle tissue lysate of Rat(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:U-87MG whole cell lysate(40ug) Lane5:HCT116 whole cell lysate(40ug)
Immunohistochemistry: Immunohistochemistry of paraffin-embedded human colon carcinoma using Cytochrome c antibody at dilution of 1:50.
Immunofluorescence analysis: Immunohistochemistry of paraffin-embedded human colon carcinoma using Cytochrome c antibody at dilution of 1:50.
Host: Rabbit
Swiss-Prot: P99999
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