Product Description
CREB (phospho-S133) polyclonal antibody is available at gentaur for next week delivery
Background: Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133.
Applications: WB IF IP
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: p-CREB (S133) polyclonal antibody detects endogenous levels of CREB protein when phosphorylated at Ser133
W4BiowMW: ~ 43 kDa
Reactivity: Human
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human CREB around the phosphorylation site of Serine 133.Alternative Name:
Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1; CREB1Western blot analysis: Western blot analysis of extracts of HeLa cells, using Phospho-CREB1-S133 antibody.
Immunohistochemistry: Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug CREB (phospho-S133) polyclonal antibody.
Immunofluorescence analysis: Immunofluorescence analysis of MCF7 cells, using CREB (phospho-S133) polyclonal antibody
Host: Rabbit
Swiss-Prot: P16220
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