Product Description
COXI polyclonal antibody is available at gentaur for next week delivery
Background: Cytochrome c oxidase subunit I, COX1 (also designated COI, MTCO1 or oxidative phosphorylation (OxPhos) Complex IV, subunit I) is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1-3) of respiratory Complex IV. Cytochrome c oxidase is a hetero-oligomeric enzyme composed of 13 subunits localized to the mitochondrial inner membrane and is the terminal enzyme complex of the electron transport chain. Complex IV catalyzes the reduction of molecular oxygen to water. The energy released is used to transport protons across the mitochondrial inner membrane. The resulting electro-chemical gradient is necessary for the synthesis of ATP. Complex IV contains 13 polypeptides; COX1, COX2 and COX3 (MTCO1-3) make up the catalytic core and are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc and VIII are nuclear-encoded.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: COXI polyclonal antibody detects endogenous levels of COXI protein.
W4BiowMW: ~ 57 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
A synthetic peptide corresponding to amino acids 433-445 of Mouse COXI.Alternative Name:
Cytochrome c oxidase subunit 1; Cytochrome c oxidase subunit I; Cytochrome oxidase subunit 1; mt-Co1; Co1; COX1; COXIWestern blot analysis: Western blot (WB) analysis of COXI pAb at 1:2000 dilution Lane1:The Brain tissue lysate of Mouse(40ug) Lane2:The Skin tissue lysate of Rat(40ug) Lane3:CT26 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug) Lane6:A375 whole cell lysate(30ug)
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: Q9MD68
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