Product Description
CD45 (Phospho-Y1216) polyclonal antibody is available at gentaur for next week delivery
Background: The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing. The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates. CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling. Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: CD45 (Phospho-Y1216) polyclonal antibody detects endogenous levels of CD45 protein only when phosphorylated at Tyr1216.
W4BiowMW: ~ 180 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human CD45 around the phosphorylation site of Tyrosine 1216.Alternative Name:
Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; PTPRC; CD45Western blot analysis: Western blot (WB) analysis of CD45 (Phospho-Y1216) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:MCF-7 whole cell lysate LaneC:PC12 whole cell lysate LaneD:AML-12 whole cell lysate
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P08575
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