CD2 Antibodies

CD2 Antibodies

Mouse anti-human CD2 antibodies

-3 clones   CLB-T11/1,6G4 (purified & FITC)

                 XIX.8 (purified, FITC & PE)

                 GJ12 (purified, FITC ,PE, & R-PE/CY5 )

-see specialty T cells stimulation clones

mouse monoclonal CD2

CAT# RDI-M1314clb     PRICE: $375.00/vial

Code: CLB-CD2 (CLB-T11)

Lot number:

Tests/vial: 200

Specificity:Cell Type Human T lymphocytes  Af MW. 50 kD.

Reactivity:The monoclonal antibody is directed against the CD2-antigen (T11-antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with all human peripheral T lymphocytes and 90% of the thymocytes. The monoclonal antibody blocks the rosette-formation of human T lymphocytes with sheep erythrocytes.

Applications:Monitoring of T-cell numbers in peripheral blood. Characterization of leukaemias and lymphomas. Identification of T-cells in tissue. Analysis of NK-cell subset.

Methods: Indirect immunofluorescence staining with analysis by FACS or    fluorescence microscopy.

Clone: CLB-163  This clone has been derived from hybridization of SP2/0 cells with spleen cells of a (BALB/c x A/J) mouse immunized with cells of a patient with Sezary Syndrome.

Mouse isotype: The monoclonal antibody is of the mouse subclass IgG1.

Source: Ascites fluid of tumor bearing BALB/c mice.

Purification: Ammoniumsulphate precipitation and ion exchange chromatography.

Packing: Each vial contains 1ml with approximately 0.1mg/ml monoclonal antibody and 10mg BSA in 20 mM TRIS, 150mM NaCI, pH 8.0.

Preservative: Merthiolate (0.001%).

Storage: Monoclonal antibodies should be stored below -18`C. Repeated thawing and freezing may reduce the antibody activity and should be avoided.

IN VITRO RESEARCH USE ONLY

mouse monoclonal CD2,conjugated to Fluorescein

Code: CLB-CD2-F (CLB-T11)

CAT# RDI-M1465clb      PRICE: $375.00/vial

Tests/vial: 100

Specificity: Cell type: Human T lymphocytes

Ag.MW.: 50 kD.

Reactivity: The monoclonal antibody is directed against the CD-2-antigen (T11-antigen), which is expressed on human Tlymphocytes. The monoclonal antibody reacts with all human peripheral T lymphocytes and 90% of the thymocyte. The monoclonal antibody blocks the rosette-formation of human T lymphocytes with sheep erythrocytes.

Methods: Direct immunofluorescence staining with analysis by FACS or fluorescence microscopy.

Clone: CLB-163This clone has been derived from hybridization of SP2/0 cells with spleen cells of a (BALB/c x A/J) mouse immunized with cells of a patient with the Sezary syndrome.

Mouse isotype: The monoclonal antibody is of the mouse subclass IgG1.

Source: Ascites fluid of tumour bearing BALB/c mice.

Purification: Ammoniumsulphate precipitation and ion exchange chromatography.

Chemical Characteristics: Molecular F/P ratio : 6.8 Weight F/P ratio : 16.5 x 10-3 Fluorescein concentration : 8.3 x 10-4 mg/ml Optical density ratio (E495/E280) at pH 7.2 : 1.27

Packing: Each vial contains 1 ml with approximately 50 ug/ml conjugated monoclonal antibody and 10 mg BSA in PBS.

Preservative: Merthiolate (0.001%).

Storage: Fluorescein labelled monoclonal antibodies should be stored 4 DEG C.Repeated thawing and freezing may reduce the antibody activity and should be avoided.

FOR IN  VITRO RESEARCH USE ONLY

Mouse ANTI-HUMAN CD2+ T LYMPHOCYTES

PRODUCT CODE :RDI-CBL140   $375.00/vial

Also available: FITC labeled cat#RDI-CBL140FT $395.00/100 Tests

                           PE labeled cat#RDI-CBL140PE $469.00/100 Tests

CLONE: XIX.8

ISOTYPE: IgG2a

SOURCE: Mouse ascitic fluid

PURIFICATION Ion exchange chromatography following ammonium sulphate precipitation.

SPECIFICITY: 50Kd MW lymphocyte surface antigen identified by monoclonal antibodies belonging to the CD2 cluster. The antigen is expressed on all peripheral blood T lymphocytes, majority of thymocytes and malignant cells of T cell origin, including T- ALL cells. Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen. Common ALL cells do not express CD2 antigen.

The antigen identified by CD2 type monoclonal antibodies has been characterised as the receptor for sheep erythrocytes and pre-treatment of lymphocytes with CD2 monoclonal antibodies inhibits E rosette formation. The antigen also functions as the receptor for the CD 58 antigen (LFA-3).

Antigen distribution: T-cells (E+)                          100%

                               Thymocytes                          95 � 3%

                               Peripheral blood lymphocytes 82 � 5%

                               NK-cells (CD16+)                50 � 10%

                               Granulocytes                          < 1%

                               Monocytes                             < 1%

                               B-cells (E-)                            < 1%

APPLICATIONS: * Identification of T lymphocytes by indirect immunofluorescence staining

                              * Leukaemia and lymphoma phenotyping

                              * Identification of normal and malignant T lymphocytes in cryostat sections of    frozen tissue

                              * Immunoprecipitation of the CD2 antigen

                              * Isolation or removal of T lymphocytes by FACS or panning techniques

REFERENCES: Leucocyte Typing III, ed. McMichael, A.J. et al . Oxford University Press (1987)

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200�g/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 30 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

For BULK (without BSA and without Preservative) use cat#RDI-CBL140-1XP  $875.00/1 milligram  Larger Sizes quoted on Request  

Mouse ANTI-HUMAN CD2 ANTIGEN

PRODUCT CODE :RDI-CBL487  $375.00/vial 200ug

Also available:  FITC labeled cat#RDI-CBL487FT      $375.00/100 Test vial

                           PE labeled    cat#RDI-CBL487PE     $469.00/100T  vial

                            R-PE/CY5     cat#RDI-CBL487PC5   $469.00/vial

CLONE   GJ12

ISOTYPE   IgG1

SOURCE  Tissue culture supernatant

PURIFICATION METHOD  Protein A affinity chromatography

SPECIFICITY : The antibody reacts with a 50 kDa lymphocyte surface molecule identified by monoclonal antibodies belonging to the CD2 cluster. The antigen is also known as sheep erythrocyte receptor and functions as a receptor for the CD58 antigen (LFA-3) through its NH2 terminal domain. The antigen is expressed on all peripheral blood T lymphocytes, the majority of thymocytes and malignant cells of T cell origin, including T ALL cells. Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen. Common ALL cells do not express CD2 antigen.

Antigen distribution:  T cells (E+)     100%

                                  Thymocytes      95 + 3%

                                 Peripheral blood lymphocytes 82 + 5%

                                 NK cells            50 + 10%

                                 B cells (E-)       < 1%

                                 Granulocytes     < 1%

                                  Monocytes        < 1%


APPLICATIONS

*Identification of T lymphocytes by indirect immunofluorescence staining

*Leukaemia and lymphoma phenotyping

*Identification of normal and malignant T lymphocytes on frozen sections

*Immunoprecipitation of the CD2 antigen

REFERENCES  Beyers, A. D. et al. Immunol Rev. III, 59-77 (1989)

                           Bierer, B. E. & Burakoff, S. J. Immunol Rev. III, 267-294 (1989)

PRESENTATION The monoclonal is presented at a concentration of 200�g/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.