Caveolin antibodies

Caveolin 1 & Caveolin-2 antibodies

Mouse anti-Caveolin-1 Monoclonal

cat# RDI-CAVEOL1abm         $438.00/vial 50ug

       RDI-CAVEOL1abm-1      $750.00/vial 150ug

Clone: 2297

isotype: mIgG1

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)

antigen: a protein fragment corresponding to amino acids 1-178 from RSV-CEF Caveolin

Use: Suitable for western blot on human, rat, mouse, dog, and chick (and rabbit) using approx 0.5ug/ml on total cell lysate of human fibroblasts. Major band =Mw 22 kDa with incubation 2-3 hour rt or overnight 4 DEG C with chemiluminescence detection. -also suitable for immunoprecipitation (native or denatured conditions), histochemistry (on human fibroblasts or rabbit lung) and immunofluorescence.

Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.

Main REF: Song, K.S. et al 1997 J. Biol Chem 272:4398-4403

                   Bickel, P.E et al 1997 J. Biol Chem 272:13793-13802

-copyright by owner

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Mouse anti-Caveolin-1 Monoclonal

cat# RDI-CAVEOL1Gabm        $438.00/vial 50ug

       RDI-CAVEOL1Gabm-1     $750.00/vial 150ug

Clone: 2234

isotype: mIgG2a

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)

antigen: a protein fragment corresponding to amino acids 1-178 from RSV-CEF Caveolin

Use: Suitable for western blot on human, rat, and chick using approx 0.1ug/ml on total cell lysate of chick embryo fibroblasts. Major band =Mw 22 kDa with incubation 2-3 hour rt or overnight 4 DEG C with chemiluminescence detection. -also suitable for immunoprecipitation (native or denatured conditions), and immunofluorescence (on human fibroblasts)

Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.

Main REF: Babitt, J. et al. 1997 J Biol Chem 272:13242-13249

                   Shaul P.W. et al, 1996 J. Biol Chem 271:6518-6522

-copyright by owner

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Rabbit anti-Caveolin-1

cat# RDI-CAVEOL1abrX   $469.00/100ul

Package: 100 ul(0.1mg/ml) of affinity purified rabbit serum with 1.0 mg/ml BSA and 0.05% sodium azide. Store at -20C. Avoid repeated freeze/thaw cycles.

Immunogen: Synthetic peptide: M(1) S G G K Y V D S E G H L Y T V P(17) C

The immunizing peptide corresponds to amino acid residues 1-17 from human caveolin-1. This sequence is completely conserved between human, mouse, canine, bovine, and rat. The immunizing peptide (Cat#RDI-CAVEOL1X-CP $156.00) is available for use in neutralization and control experiments.

Use: This polyclonal antibody detects caveolin-1 from canine, hamster, mouse and rat tissues and cells. It has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects an ~22 kDa protein representing caveolin-1 from rat heart protein extract

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.

References: Cytokine Growth Factor Rev., 2:41-51, 2001.

                    J. Cell. Sci., 114:1397-1408, 2001.

                    Traffic, 1:212-217, 2001.

Background: Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types

For Research Use Only

Rabbit anti-Caveolin-2

cat# RDI-CAVEOL2abrX   $469.00/100ul

Package: 100 ul(0.1mg/ml) of affinity purified rabbit serum with 1.0 mg/ml BSA and 0.05% sodium azide. Store at -20C.

Avoid repeated freeze/thaw cycles.

Immunogen: Synthetic peptide:   M(1) G L E T E K A D V Q L F M A D D A Y(19) C

The immunizing peptide corresponds to amino acid residues 1-19 of human caveolin-2. This sequence is completely conserved in mouse and rat caveolin-2. The immunizing peptide (Cat#RDI-CAVEOL2X-CP $188.00) is available for use in neutralization and control experiments.

Use: detects caveolin-2 from rat tissues. This antibody does not detect caveolin-1 or -3. This polyclonal has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a ~21 kDa protein representing caveolin-2 from rat heart homogenate. -reactivity expected with human and mouse based on immunogen sequence homology.

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.

References: J. Biol. Chem., 272: 29337-29346, 1997.

Background: Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types