Product Description
Cattle HB(Hemoglobin) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: Hgb; Haemoglobin; Heterotetramer(αβ)2
Species: Cattle
Assay Type: Competitive Inhibition
Sensitivity: 5.57 μg/mL
Standard: 1000 μg/mL
Detection Range: 15.63-1000 μg/mL
Sample Type: Serum, plasma, erythrocyte lysates and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Metabolic pathway;Infection immunity;Hematology;Neuro science;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hemoglobin(HB) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemoglobin(HB). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin(HB) in the samples is then determined by comparing the OD of the samples to the standard curve.
