Product Description
Cattle a1AGP(Alpha-1-Acid Glycoprotein) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: alpha1-AGP，AGP1; a1-AGP; ORM1; OMD 1; Orosomucoid 1
Species: Cattle
Assay Type: Sandwich
Sensitivity: 7 ng/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 ng/mL
Sample Type: serum, plasma, urine and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Infection immunity;Hepatology;Gastroenterology;Immunodeficiency;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Acid Glycoprotein(a1AGP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Acid Glycoprotein(a1AGP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Acid Glycoprotein(a1AGP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Acid Glycoprotein(a1AGP) in the samples is then determined by comparing the OD of the samples to the standard curve.
