CASP9 (phospho-S144) polyclonal antibody

Product Description

CASP9 (phospho-S144) polyclonal antibody is available at gentaur for next week delivery

Background: Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response. Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis.

Applications:  WB

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  CASP9(Phospho-S144) polyclonal antibody detects endogenous levels of CASP9 protein only when phosphorylated at Ser144.

W4BiowMW:  ~ 47 kDa

Reactivity: Human

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic phosphopeptide derived from human CASP9 around the phosphorylation site of Serine 144.

Alternative Name:

Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6; Caspase-9 subunit p35; Caspase-9 subunit p10; CASP9; MCH6

Western blot analysis:  Western blot (WB) analysis of CASP9(Phospho-S144) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:A375 whole cell lysate(40ug)

Immunohistochemistry:

Immunofluorescence analysis:

Host:  Rabbit

Swiss-Prot: P55211

 

 

 

 

"