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  • Calpain 2 polyclonal antibody
  • Calpain 2 polyclonal antibody
  • Calpain 2 polyclonal antibody

    Calpain 2 polyclonal antibody

    bioworld

    MSRP:
    Now: €350.00
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    SKU:
    CAS7518
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    Product Description

    Calpain 2 polyclonal antibody is available at gentaur for next week delivery

    Background: Calpain is a calcium-dependent thiol proteinase that is functionally active as a heterodimer composed of a small regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation. The regulation of calpain in vivo is the subject of many current studies, which suggest that proteolytic activity is regulated post-transcriptionally by mechanisms such as calcium requirements, subcellular localization of the heterodimer, phosphorylation via the EGFR-Erk signaling cascade, endogenous inhibitors (calpastatin) and autoproteolytic cleavage. Calpastatin negatively regulates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28. Calpain influences cell migration by modifying rather than degrading its substrates responsible for cell adhesion and cytoskeletal arrangement. Control of calpain activity has caught the attention of drug development since limiting its activity could mute invasiveness of tumors or chronic inflammation.

    Applications:  WB IP

    Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).

    Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

    Specificity:  Calpain 2 polyclonal antibody detects endogenous levels of Calpain 2 protein.

    W4BiowMW:  ~ 80 kDa

    Reactivity: Human,Rat,Mouse

    Note:  For research use only, not for use in diagnostic procedure.

    Immunogen:

    Recombinant protein of human Calpain 2.

    Alternative Name:

    Calpain-2 catalytic subunit; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain; M-calpain; CAPN2; CANPL2

    Western blot analysis:  Western blot (WB) analysis of Calpain 2 polyclonal antibody at 1:5000 dilution Lane1:Hela whole cell lysate(30ug) Lane2:A375 whole cell lysate(30ug) Lane3:The Skin tissue lysate of Rat(40ug) Lane4:SGC7901 whole cell lysate(40ug) Lane5:P12 whole cell lysate(40ug) Lane6:AML-12 whole cell lysate(40ug)

    Immunohistochemistry: Immunoprecipitation - Calpain 2 Polyclonal Antibody

    Immunofluorescence analysis:

    Host:  Rabbit

    Swiss-Prot: P17655

     

     

     

     

    "

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