Product Description
Calpain 2 (K354) polyclonal antibody is available at gentaur for next week delivery
Background: Mu Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names.
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Calpain 2 (K354) polyclonal antibody detects endogenous levels of Calpain-2 catalytic subunit protein.
W4BiowMW: ~ 80 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to amino acids 320-370 of Human Calpain 2.Alternative Name:
Calpain-2 catalytic subunit; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain; M-calpain; CANPL2; CAPN2Western blot analysis: Western blot (WB) analysis of Calpain 2 (K354) pAb at 1:500 dilution Lane1:Beas-2B whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:PC3 whole cell lysate(40ug) Lane4:The Kidney tissue lysate of Mouse(40ug) Lane5:The Kidney tissue lysate of Rat(40ug)
Immunohistochemistry: Immunohistochemistry (IHC) analyzes of Calpain 2 (K354) pAb in paraffin-embedded human brain tissue.
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P17655
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