C/EBP-β (phospho-T235/188) polyclonal antibody

Product Description

C/EBP-β (phospho-T235/188) polyclonal antibody is available at gentaur for next week delivery

Background: C/EBP β is a member of the C/EBP transcription factor family. The C/EBP β gene encodes several isoforms, which have truncated transcription activation domains by the alternative translational initiation at multipe AUG start sites. Initiation of translation at the in-frame AUGs forms four C/EBP β isoforms. C/EBP β is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein(LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C/EBP β contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C/EBP β gene that has direct implication for acute phase response in liver cells.The 35 kDa C/EBP β form requires phosphorylation for its DNA binding ability, and increased binding of C/EBP β isoforms during acute-phase reaction occurs through its upregulation and structural modification.

Applications:  WB IHC

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  p-C/EBP-β (T235/188) polyclonal antibody detects endogenous levels of human LAP only when phosphorylated at Thr235, mouse and rat LAP only when phosphorylated at Thr188, and LIP only when phosphorylated at Thr37.

W4BiowMW:  ~ 38 kDa

Reactivity: Human

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic phosphopeptide derived from human C/EBP-β around the phosphorylation site of Threonine 235.

Alternative Name:

CCAAT/enhancer-binding protein beta; C/EBP β; C/EBPβ; C/EBP beta; Liver activator protein; LAP; Liver-enriched inhibitory protein; LIP; Nuclear factor NF-IL6; Transcription factor 5; TCF-5; CEBPB; TCF5; ORF Names: PP9092

Western blot analysis:  Western blot (WB) analysis of C/EBP-β (phospho-T235/188) pAb at 1:500 dilution Lane1:MCF-7 treated with insulin(500ng/ml,5min) whole cell lysate(40ug) Lane2:MCF-7 treated with insulin(500ng/ml,0min) whole cell lysate (40ug)

Immunohistochemistry: Immunohistochemistry (IHC) analyzes of p-C/EBP-β (T235/188) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunofluorescence analysis:

Host:  Rabbit

Swiss-Prot: P17676

 

 

 

 

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