Product Description
C/EBP epsilon (Phospho-T74) polyclonal antibody is available at gentaur for next week delivery
Background: C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic α helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP α appears to function exclusively in terminally-differentiated, growth-arrested cells. Additional family members include C/EBP β, C/EBP γ, C/EBP δ and C/EBP ε, all of which exhibit similar DNA-binding specificities and affinities to C/EBP α. Furthermore, C/EBP β and C/EBP δ readily form heterodimers both with each other as well as with C/EBP α.
Applications: WB IHC IF
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: C/EBP epsilon (Phospho-T74) polyclonal antibody detects endogenous levels of C/EBP epsilon protein only when phosphorylated at Thr74.
W4BiowMW: ~ 34 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human C/EBP epsilon around the phosphorylation site of Threonine 74.Alternative Name:
CCAAT/enhancer-binding protein epsilon; C/EBP epsilon; CEBPE; C/EBPεWestern blot analysis: Western blot (WB) analysis of C/EBP epsilon (Phospho-T74) polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:The Liver tissue lysate of Rat LaneC:Raw264.7 whole cell lysate LaneD:The Liver tissue lysate of Rat
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: Q15744
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