Product Description
BRK (Phospho-Y342) polyclonal antibody is available at gentaur for next week delivery
Background: The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants.
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: BRK (Phospho-Y342) polyclonal antibody detects endogenous levels of BRK protein only when phosphorylated at Tyr342.
W4BiowMW: ~ 52 kDa
Reactivity: Human,Mouse
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human BRK around the phosphorylation site of Tyrosine 342.Alternative Name:
Protein-tyrosine kinase 6; Breast tumor kinase; Tyrosine-protein kinase BRK; PTK6; BRKWestern blot analysis: Western blot (WB) analysis of BRK (Phospho-Y342) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:HCT116 whole cell lysate LaneC:NIH3T3 whole cell lysate
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: Q13882
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