Monoclonal mouse anti-alcohol dehydrogenase (Human)

CAT#: RDI-TRK4AD1-1E5

PRICE: $300.00/mg or $250.00/mg 2 or more


Clone: ADH 1E5
These clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of a Balb/c mice immunized with purified alcholol dehydrogenase from human liver.

Specificity: Alcohol dehydrogenase (ADH),human

Reactivity: MoAb ADH 1E5 reacts with ADH with Kd=10-10M. MoAb ADH 1E5 does not influeince the oxidoreductase activity. MoAb 1E5 do not cross-react with ADH from rat and rabbit. Specific antibody titer in indirect ELISA is 1/1,000,000.

Mouse isotype: All IgG1

Application: Immynocytochemistry analysis of hepatocyte dif ferentiation.

Purification: Purified by chromatography on protein A Sepharose.

Purity is tested by electrophoresis

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 1 milligram of murine monoclonal antibody dissolved in phosphate buffered saline containing 0.1% sodium azide. We recommend that each laboratory determine an optimum working titer for use in its particular application.

Storage: Purified monoclonal antibodies are generally stable for period at 4 0C. However repeated warming to room temperature and re-cooling may result in loss of activity and hence working titre.

FOR IN VITRO RESEARCH USE ONLY. NOT FOR USE IN OR ON HUMANS OR ANIMALS-

NOT FOR DIAGNOSTICS. Not responsible for any Patent infringements with use or derivation of these products. Contains sodium azide (0.1%)-observe proper precautions.

Catalog#: RDI-ALDEYabr $200.00/vial

Also available HRP conjugated: cat#RDI-ALDEY-HRP $200.00/20mg

Biotin conjugated cat#RDI-ALDEY-BT $200.00/10mg

Size: 50 mg (lyophilized)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,pH 7.2

Stablizer: None

Preservative: 0.01% (w/v) Sodium Azide

Antigen: Yeast alcohol dehydrogenase

Applications: suitable for immunoblotting (western or dot blot 141kDa band under non reducing conditions, and 35kDa under reducing conditions) and ELSIA. Minimal cross expected with mammalian enzymes.

Recommended Dilutions: Not determined. Optimal titers for applications should be determined by the researcher.

Storage Conditions: Store vial at 4'C prior to restoration. Re-store with 5.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4'C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20'C or below. Avoid cycles of freezing and thawing. Expiration date is one (1)year from date of reconstituion.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.