Mab to Adipophilin
cat#RDI-PRO651102 $250.00/vial $225.00/vial 3 or more
also available purified cat#RDI-PRO610102 $300.00/50ug $250.00/vial 3+
FITC conjugated cat#RDI-PRO614102 $350.00/vial 0.25ml
Clone AP125
Category Mouse monoclonal
Immunoglobulin Class IgG1
Form/Purification Hybridoma culture supernatant
Antigen synthetic peptide (aa 5-27 from N-terminus of human adipophilin)
Clinical Application/
Specificity New specific marker for liposarcoma. Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines, inducible by etomixir **
Polypeptide Reacting: Adipophilin, Mr 48,100.13 (calculated from aa sequence data) apparent Mr 52,000 (after SDS-PAGE); pI 6.72
Immunolocalization: Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver, adipocytes are negative. Specific detection of liposarcoma. Also positively stained are lipid storing CD68 positive macrophages
Antigen Recognizied: Human, rat, dog (negative with bovine and mouse)
Reactivities on cultured Cell Lines: Caco, PLC, HaCat, SV80, RD 125, Huvec (human umbilical cord endothelial), RV, PC-12 (rat adrenal gland), MDCK; negative with glioma
Application o Immunoblotting o Immunohistochemistry on frozen tissue, paraffin embedded after high t temp/microwave treatment
Working Dilution: Ready to Use for immunohistochemistry (unpurified)
-approx 1:10 for purified (50ug in 1ml)
Storage At 2-8 DEC C
Volume 5 ml (unpurified) or 50ug purified or 0.25ml (50ug FITC conjugated)
References
Hope, R.G. and McLauchlan J. Sequence motifs required fior lipd droplet association and protein stability are unique to the hepatitis C virus core protein. J General Virology 81, 1913-1925 (2000)
Heid, HW,et al, Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res 294:309-321 (1998)
Heid HW, Schnolzer M and TW Keenan. Adipocyte differentiation-related protein is secreted into milk as a constituent of milk lipid globule membrane. Biochem J 320:1025-1030 (1996)
Mrozinzki, J et al, Adipophilin and perilipin, two lipid droplet-associated proteins, in the pathohistological diagnosis of human soft tissue carcinomas. Abstract from AEK meeting 1999, German Cancer Research Center, J Cancer Res & Clin Oncol 125 suppl (1999)
Steiner S, Wahl D, Mangold BL, Robiron R, Raymackers J, Mehens L, Anderson NL, and Cordier A. Induction of the adipose differentiaion-related protein in the liver of rats. Biochem Biophys Res 218:777-782 (1996)
For In Vitro Research Use Only
Guinea Pig Pab to Adipophilin (HUMAN/MOUSE/Canine/RAT/Bovine)
human homologue to mouse adipocyte differentiation-related protein (ADRP)
cat#RDI-PROGP40 $350.00
Category Guinea pig polyclonal (GP40, mN1)
Purification / Form Whole antiserum
Immunogen Synthetic peptide (N-terminal aa 1-25 of murine adipophilin)
Description/Specificity New specific marker for liposarcoma. Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir*.
Polypeptide reacting: Adipophilin, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72
Immunolocalization Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Specific detection of liposarcoma.
Also positively stained are lipid-storing CD 68-positive macrophages.
Antigen recognized species Human, mouse, rat, dog, bovine
(tested so far)
Reactivities on Cultured Cell Caco, PLC, HaCat, SV80, RD 125, Huvec
Lines (tested so far) (human umbilical cord endothelia), RV, PC-12 (rat adrenal gland),
MDCK; negative with glioma
Application Immunoblotting (Western blot)
Immunohistochemistry of frozen tissue, paraffin-embedded tissue
after microwave treatment
Cytological material
Working Dilution 1:100 for immunohistochemistry 1:2000 for immunoblotting
Incubation Time 1 h at room temperature or over night at 2-8°C
Storage At 2-8°C
Quantity 100µL; contains 0.09% sodium azide
References
Heid HW, Moll R, Schwetlick I, Rackwitz HR and Keenan TW: Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res 294:309-321 (1998)
Heid HW, Schnölzer M and TW Keenan. Adipocyte differentiation-related protein is secreted into milk as a constituent of milk lipid globule membrane. Biochem J 320:1025-1030 (1996)
Mrozinzki J, SchwetlicK I, Heid HW, Winter-Simanowski S, Holzbach A, Hinze R, Franke WW and Moll R. Adipophilin and perilipin, two lipid droplet-associated proteins, in the pathohistological differential diagnosis of human soft tissue sarcomas. Abstract from AEK meeting 1999, German Cancer Research Center. J.Cancer Res.& Clin.Oncol. 125 suppl. (1999)
Steiner S, Wahl D, Mangold BL, Robiron R, Raymackers J, Mehens L, Anderson NL, and Cordier A. Induction of the adipocyte differentiation-related protein in the liver of rats. Biochem Biophys Res 218:777-782 (1996)
Hope R.G. and McLauchlan J. Sequence motifs required for lipid droplet association and protein stability are unique to the hepatitis C virus core protein. J. General Virology 81, 1913-1925 (2000)
For Research Use Only