Product Description
BIK (Phospho-S35) polyclonal antibody is available at gentaur for next week delivery
Background: Bik/Nbk (Bcl-2-interacting killer/natural born killer) is a potent pro-apoptotic protein belonging to a group of Bcl-2 family members that includes Bad, Bid, Bim, Hrk, and Noxa, containing a BH3 domain but lacking other conserved domains, BH1 or BH2. Functionally, Bik is able to bind to and antagonize anti-apoptotic Bcl-2 family members including Bcl-2, Bcl-xL, and viral homologs E1B-19K and EBV-BHFR1. The BH3 domain of Bik is essential for its apoptotic activity and interaction with survival proteins. Phosphorylation of Bik is correlated with an increase in its pro-apoptotic activity.
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: BIK (Phospho-S35) polyclonal antibody detects endogenous levels of BIK protein only when phosphorylated at Ser35.
W4BiowMW: ~ 18 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human BIK around the phosphorylation site of Serine 35.Alternative Name:
Bcl-2-interacting killer; Apoptosis inducer NBK; BIP1; BP4; BIK; NBKWestern blot analysis: Western blot (WB) analysis of BIK (Phospho-S35) polyclonal antibody at 1:500 dilution LaneA:The Embryo tissue lysate of Mouse LaneB:The Ovary tissue lysate of Rat LaneC:HEK293T starved with EGF whole cell lysate LaneD:HEK293T whole cell lysate
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: Q13323
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