Product Description
BID p11 polyclonal antibody is available at gentaur for next week delivery
Background: Bid is a pro-apoptotic “BH3 domain-only” member of the Bcl-2 family originally discovered to interact with both the anti-apoptotic family member Bcl-2 and the pro-apoptotic protein Bax. Bid is normally localized in the cytosolic fraction of cells as an inactive precursor and is cleaved at Asp60 by caspase-8 during Fas signaling, leading to translocation of the carboxyl terminal p15 fragment (tBid) to the mitochondrial outer membrane. Translocation of Bid is associated with release of cytochrome c from the mitochondria, leading to complex formation with Apaf-1 and caspase-9 and resulting in caspase-9 activation. Thus, Bid relays an apoptotic signal from the cell surface to the mitochondria triggering caspase activation.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: BID p11 polyclonal antibody detects endogenous levels of BID p11 protein.
W4BiowMW: ~ 21 kDa
Reactivity: Human
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to Human BID p11.Alternative Name:
BH3-interacting domain death agonist; p22 BID; BID; p15 BID; p13 BID; p11 BIDWestern blot analysis: Western blot (WB) analysis of BID p11 polyclonal antibody at 1:500 dilution LaneA:OPM2 whole cell lysate LaneB:OPM2 treated with DRB(50μM) for 2 hours whole cell lysate LaneC:OPM2 treated with DRB(50μM) for 4 hours whole cell lysate LaneD:OPM2 treated with DRB(50μM) for 6 hours whole cell lysate
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P55957
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