BID (I72) polyclonal antibody

Product Description

BID (I72) polyclonal antibody is available at gentaur for next week delivery

Background: BID, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.

Applications:  WB IHC

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  BID (I72) polyclonal antibody detects endogenous levels of BID protein.

W4BiowMW:  ~ 22 kDa

Reactivity: Human

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic peptide, corresponding to amino acids 50-100 of Human BID.

Alternative Name:

BH3-interacting domain death agonist; p22 BID; BID; BH3-interacting domain death agonist p15; p15 BID; BH3-interacting domain death agonist p13; p13 BID;,BH3-interacting domain death agonist p11; p11 BID; BID

Western blot analysis:  Western blot (WB) analysis of BID (I72) pAb at 1:1000 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:SK-OVCAR3 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug)

Immunohistochemistry: Immunohistochemistry (IHC) analyzes of BID (I72) pAb in paraffin-embedded human brain tissue.

Immunofluorescence analysis:

Host:  Rabbit

Swiss-Prot: P55957

 

 

 

 

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