Product Description
BCL2 polyclonal antibody is available at gentaur for next week delivery
Background: Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release. It has been implicated in modulating mitochondrial calcium homeostasis and proton flux . Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74, and Ser87. It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events. Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes. Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: BCL2 polyclonal antibody detects endogenous levels of BCL2 protein.
W4BiowMW: ~ 30 kDa
Reactivity: Human,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant protein of human BCL2.Alternative Name:
Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; MYCWestern blot analysis: Western blot (WB) analysis of BCL2 polyclonal antibody at 1:10000 dilution Lane1:A375 whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:The Thymus tissue lysate of Rat(40ug) Lane5:MCF-7 whole cell lysate(40ug)
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P10415
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