Product Description
Bax polyclonal antibody is available at gentaur for next week delivery
Background: The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax p21 (for Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
Applications: WB IHC IP
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Bax polyclonal antibody detects endogenous levels of Bax protein.
W4BiowMW: ~ 20 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant full length Human Bax.Alternative Name:
Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BAX; BCL2L4Western blot analysis: Western blot analysis of extracts of various cell lines, using BAX antibody at 1:1000 dilution. Lysates/proteins: 40ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
Immunohistochemistry: Western blot analysis of extracts of various cell lines, using BAX antibody at 1:1000 dilution. Lysates/proteins: 40ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
Immunofluorescence analysis: Immunohistochemistry of paraffin-embedded mouse testis using BAX antibody at dilution of 1:100 (40x lens).
Host: Rabbit
Swiss-Prot: Q07812
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