Product Description
ATP6V1H polyclonal antibody is available at gentaur for next week delivery
Background: Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis and an integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, c’ and c”) make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. The H subunit of V-ATPase, also designated SDF is comprised of two polypeptides derived from the same gene. This regulatory subunit plays a critical role in the functional coupling of ATP hydrolysis activity to proton transport in the V-ATPase pump.
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: ATP6V1H polyclonal antibody detects endogenous levels of ATP6V1H protein.
W4BiowMW: ~ 55 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to Human ATP6V1H.Alternative Name:
V-type proton ATPase subunit H; V-ATPase subunit H; Nef-binding protein 1; NBP1; Protein VMA13 homolog; V-ATPase 50/57 kDa subunits; Vacuolar proton pump subunit H; Vacuolar proton pump subunit SFD; ATP6V1H; CGI-11Western blot analysis: Western blot (WB) analysis of ATP6V1H polyclonal antibody at 1:500 dilution LaneA:H9C2 whole cell lysate LaneB:MEF whole cell lysate LaneC:A549 whole cell lysate LaneD:HepG2 whole cell lysate
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: Q9UI12
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