Product Description
ATP5H (D146) polyclonal antibody is available at gentaur for next week delivery
Background: ATP5H (ATP synthase, H+ transporting, mitochondrial Fo complex, subunit δ), also known as ATPQ, is a 161 amino acid protein that belongs to the ATPase δ subunit family. F-type ATPases, such as ATP5H, consist of two linked components: CF1, a soluble catalytic core that consists of five different subunits (α, β, γ, δ and ε), and CF0, a membrane proton channel that contains nine subunits (α, β, χ, δ, ε, φ, γ, F6 and 8). ATP5H encodes the δ subunit of the F0 complex. ATP5H produces ATP from ADP in the presence of a proton gradient across the membrane, which is generated by electron transport complexes of the respiratory chain. Localizing to mitochondrial inner membrane,
Applications: WB IHC IP
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: ATP5H (D146) polyclonal antibody detects endogenous levels of ATP5H protein.
W4BiowMW: ~ 18 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to amino acids 121-170 of Human ATP5H.Alternative Name:
ATP synthase subunit d, mitochondrial; ATPase subunit d; ATP5H; ORF Names: My032Western blot analysis: Western blot (WB) analysis of ATP5H (D146) pAb at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:H1792 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug)
Immunohistochemistry: Immunohistochemistry (IHC) analyzes of ATP5H (D146) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Immunofluorescence analysis: Immunoprecipitation of HEK293T cell lysate using ATP5H (D146) polyclonal antibody (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (lane 4 ).Lane 1 is 30% input.The western blot was probed using ATP5H (D146) #BS3812.“↑”（supernatant）;“↓(deposition）
Host: Rabbit
Swiss-Prot: O75947
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