Product Description
APAF1 polyclonal antibody is available at gentaur for next week delivery
Background: The mammalian homologs of the Ced-4 proteins, Apaf-1 (Ced-4), Nod1 (CARD4), and Nod2 contain a caspase recruitment domain (CARD) and a putative nucleotide binding domain, signified by a consensus Walker's A box (P-loop) and B box (Mg2+-binding site). Nod1 contains a putative regulatory domain and multiple leucine-rich repeats. Nod1 is a member of a growing family of intracellular proteins which share structural homology to the apoptosis regulator Apaf-1. Nod1 associates with the CARD-containing kinase RICK and activates NFκB. The self-association of Nod1 mediates proximity of RICK and the interaction of RICK with IKKγ. In addition, Nod-1 binds to multiple caspases with long prodomains, but specifically activates caspase-9 and promotes caspase-9-induced apoptosis. Nod2 is composed of two N-terminal CARDs, a nucleotide-binding domain, and multiple C-terminal leucine-rich repeats. The expression of Nod2 is highly restricted to monocytes, and activates NFκB in response to bacterial lipopoly-saccharides.
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: APAF1 polyclonal antibody detects endogenous levels of APAF1 protein.
W4BiowMW: ~ 135 kDa
Reactivity: Human
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
A synthetic peptide corresponding to residues in Human APAF1.Alternative Name:
Apoptotic protease-activating factor 1; APAF-1; APAF1; KIAA0413Western blot analysis: Western blot (WB) analysis of APAF1 pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug)
Immunohistochemistry: Immunohistochemistry (IHC) analyzes of APAF1 pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50,showing cytoplasm staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: O14727
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