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  • Anti-HSP70 (P.Falciparum) Antibody | Gentaur
  • Anti-HSP70 (P.Falciparum) Antibody
  • Anti-HSP70 (P.Falciparum) Antibody

    Anti-HSP70 (P.Falciparum) Antibody | Gentaur

    Gentaur

    MSRP:
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    SKU:
    400-SPC-186D-GEN
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    Product Description

    Anti-HSP70 (P.Falciparum) Antibody

    Species Reactivity Dog, Human, Non-human primate

    Host/Isotype Mouse / IgG1, kappa

    Class Monoclonal

    Type Antibody

    Clone 1B2hsp70

    Conjugate UnconjugatedSee Additional Formats

    Form Liquid

    Concentration 0.5 mg/mL

    Purification Affinity chromatography

    Storage buffer PBS, pH 7.2

    Contains 0.09% sodium azide

    Storage conditions 4° C

    RRID AB_2865351

    Product Specific Information

    Description: The monoclonal antibody 1B2hsp70 recognizes HSP (heat shock protein) 70. Hsps are highly conserved molecular chaperones that associate with proteins to stabilize or aid in the folding of new proteins or denatured proteins following chemical or physical stress. Hsp expression is both constitutive and inducible depending on the cell and its surrounding conditions. Hsps have been known to be upregulated in conditions such as cancer, ischemic injury, inflammatory, and demyelinating disease.

    This antibody recognizes the Hsp70 family member 1A found in the cytoplasm and nucleus of numerous cell types. Given the high degree of conservation of this protein across species, the 1B2hsp70 clone is expected to recognize canine and monkey HSP70 1a.

    Applications Reported: This 1B2hsp70 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.

    Applications Tested: This 1B2hsp70 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded tissue using both low and high pH antigen retrieval at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

    Purity: Greater than 90%, as determined by SDS-PAGE.

    Aggregation: Less than 10%, as determined by HPLC.

    Filtration: 0.2 µm post-manufacturing filtered.

    Target Information

    HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined.

    The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria.

    Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles.

    HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

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