Product Description
Alpha-actin polyclonal antibody is available at gentaur for next week delivery
Background: Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells. Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin. The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments. Research studies have shown that actin is hyperphosphorylated in primary breast tumors. Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies. Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis.
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Alpha-actin polyclonal antibody detects endogenous levels of Alpha-actin protein.
W4BiowMW: ~ 42 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to Human Alpha-actin.Alternative Name:
Actin, alpha cardiac muscle 1; Alpha-cardiac actin; Actin, alpha cardiac muscle 1, intermediate form; ACTC1; ACTCWestern blot analysis: Western blot (WB) analysis of Alpha-actin polyclonal antibody at 1:500 dilution LaneA:C6 whole cell lysate LaneB:AML-12 whole cell lysate
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P68032
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