AKT1/2 (Phospho-T308/T309) polyclonal antibody

Product Description

AKT1/2 (Phospho-T308/T309) polyclonal antibody is available at gentaur for next week delivery

Background: AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.

Applications:  WB

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  AKT1/2 (Phospho-T308/T309) polyclonal antibody detects endogenous levels of AKT1/2 protein only when phosphorylated at Thr308/Thr309.

W4BiowMW:  ~ 55 kDa

Reactivity: Human,Mouse,Rat

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic phosphopeptide derived from human AKT1/2 around the phosphorylation site of Threonine 308/Threonine 309.

Alternative Name:

AKT1; AKT1; AKT2; AKT3; MGC9965; Oncogene AKT1; PKB alpha; PKB; PKB beta; PKBalpha; PRKBA; PRKBB; PRKBG; Protein kinase B; Protein kinase B beta; Protein kinase B gamma; Proto-oncogene c-Akt; RAC alpha; RAC; RAC gamma; RAC PK alpha; RAC PK beta; RAC

Western blot analysis:  Western blot (WB) analysis of AKT1/2 (Phospho-T308/309) polyclonal antibody at 1:500 dilution LaneA:PC3 whole cell lysate LaneB:MCF-7 whole cell lysate LaneC:PC12 whole cell lysate LaneD:BV2 whole cell lysate

Immunohistochemistry:

Immunofluorescence analysis:

Host:  Rabbit

Swiss-Prot: P31749/P31751

 

 

 

 

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