Product Description
AKT (Phospho-Y474) polyclonal antibody is available at gentaur for next week delivery
Background: AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: AKT (Phospho-Y474) polyclonal antibody detects endogenous levels of AKT protein only when phosphorylated at Tyr474.
W4BiowMW: ~ 60 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human AKT around the phosphorylation site of Tyrosine 474.Alternative Name:
RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RACWestern blot analysis: Western blot (WB) analysis of AKT (Phospho-Y474) polyclonal antibody at 1:500 dilution LaneA:Zebrafish
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P31749
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