AKT (P467) polyclonal antibody

Product Description

AKT (P467) polyclonal antibody is available at gentaur for next week delivery

Background: AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.

Applications:  WB IHC

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  AKT (P467) polyclonal antibody detects endogenous levels of total AKT protein.

W4BiowMW:  ~ 60 kDa

Reactivity: Human,Mouse,Rat

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic peptide, corresponding to amino acids 440-490 of Human AKT1.

Alternative Name:

RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC; PKBα; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT2; PKBβ; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2; AKT3; PKBG; PKBγ

Western blot analysis:  Western blot (WB) analysis of AKT (P467) pAb at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:MEF whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug)

Immunohistochemistry: Immunohistochemistry (IHC) analyzes of AKT (P467) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunofluorescence analysis: Immunohistochemistry (IHC) analyzes of AKT (P467) pAb in paraffin-embedded human stomach carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Host:  Rabbit

Swiss-Prot: P31749/P31751/Q9Y243

 

 

 

 

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