Product Description
AGRN polyclonal antibody is available at gentaur for next week delivery
Background: Agrin is a molecule that resides in the basal lamina of muscle cells and directs key events in post synaptic differentiation. Most notably, Agrin is responsible for the clustering of acetylcholine receptors (AChRs) on the cell surface and their localization to the neuromuscular junction. Several Agrin variants have been identified which arise from alternative mRNA splicings. Agrin splice forms having inserts at two sites in the carboxy terminus designated “y” and “z” display a high affinity for AChRs, while splice forms lacking these inserts associate with AChRs weakly. Muscle alpha-dystroglycan has been postulated to be the receptor for the clustering activity of agrin; however, this is a point of contention. Tyrosine phosphorylation has been implicated as a required early step in AChR aggregation. Interestingly, a unique receptor tyrosine kinase, designated MuSK, has been discovered that interacts with Agrin and is specifically localized to developing muscle .
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: AGRN polyclonal antibody detects endogenous levels of AGRN protein.
W4BiowMW: ~ 217 kDa
Reactivity: Human,Rat,Mouse
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to Human AGRNAlternative Name:
Agrin; Cleaved into the following 4 chains: Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment; C90; Agrin C-terminal 22 kDa fragment; C22; AGRN; AGRINWestern blot analysis: Western blot (WB) analysis of AGRN polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40µg) Lane2:H1792 whole cell lysate(40µg) Lane3:PC12 whole cell lysate(40µg) Lane4:AML-12 whole cell lysate(40µg)
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: O00468
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