Product Description
β-Actin (4D3) monoclonal antibody-HRP is available at gentaur for next week delivery
Background: Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells.
Applications: WB
Purification&Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: β-Actin (4D3) detects endogenous levels of β-actin protein.
W4BiowMW: ~ 42 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant full length Human β-Actin.Alternative Name:
Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed; ACTB; Actin-β; Beta actin;Western blot analysis: Western blot (WB) analysis of β-Actin (4D3) mAb-HRP at 1:2000/1:5000/1:10000 dilution Lane1:Hela whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug)
Immunohistochemistry:
Immunofluorescence analysis:
Host: Mouse
Swiss-Prot: N/A
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