Product Description
β-Actin (4D3) monoclonal antibody is available at gentaur for next week delivery
Background: Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells.
Applications: WB IHC IF
Purification&Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: β-Actin (4D3) detects endogenous levels of β-actin protein.
W4BiowMW: ~ 42 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant full length Human β-Actin.Alternative Name:
Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed; ACTB; Actin-β; Beta actin;Western blot analysis: Western blot (WB) analysis of β-Actin (4D3) mAb at 1:5000-1:20000 dilution Lane1:The Lung tissue lysate of Mouse(20ug) Lane2:The Lung tissue lysate of Rat(20ug) Lane3:H9C2 whole cell lysate(20ug) Lane4:CT26 whole cell lysate(20ug) Lane5:L02 whole cell lysate(20ug) Lane6:HEK293T whole cell lysate(20ug)
Immunohistochemistry:
Immunofluorescence analysis: IF image of BS6007M stained A549 cells.The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody β-Actin (4D3) #BS6007M(1:200) at 5µg/ml overnight at +4°C. The secondary antibody (Red) was Goat Anti-Mouse IgG (H+L) Rhodamine (TRITC)#BS11502 used at a 1/1000 dilution for 1h. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).
Host: Mouse
Swiss-Prot: N/A
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