AATF polyclonal antibody

Product Description

AATF polyclonal antibody is available at gentaur for next week delivery

Background: Che-1 (protein AATF, apoptosis-antagonizing transcription factor) is a widely expressed nuclear protein that belongs to the AATF family. Hyperphosphorylated during the G1/S phase transition, Che-1 may function as a general inhibitor of the histone deacetylase HDAC1. Che-1 binding to the pocket region of Rb may displace HDAC1 from Rb/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Displacement of HDAC1 from Sp1 bound to the p21 promoter leads to increased expression of Che-1. It also antagonizes PAR4 (prostate apoptosis response 4) mediated induction of aberrant amyloid peptide production in Alzheimer disease (AD; presenile and senile dementia). PAR4 is a leucine zipper protein that is pro-apoptotic and associated with neuronal degeneration in AD. Che-1 interaction with PAR4 suggests that it might directly or indirectly participate in regulation of PAR4 activity. Che-1 also co-localizes with PAR4 in both cytoplasmic and nuclear compartments, and it interacts directly and selectively with PAR4 via the leucine zipper domain in neural cells.

Applications:  WB IHC IF

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  AATF polyclonal antibody detects endogenous levels of AATF protein.

W4BiowMW:  ~ 63 kDa

Reactivity: Human

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic peptide, corresponding to Human AATF.

Alternative Name:

Protein AATF; Apoptosis-antagonizing transcription factor; Rb-binding protein Che-1; AATF; CHE1; DED; HSPC277

Western blot analysis:  Western blot analysis of extracts of Hela cell line, using AATF polyclonal antibody.

Immunohistochemistry:

Immunofluorescence analysis:

Host:  Rabbit

Swiss-Prot: Q9NY61

 

 

 

 

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