Product Description
14-3-3 θ/YWHAQ (phospho-S232) polyclonal antibody is available at gentaur for next week delivery
Background: Protein interactions with 14-3-3 show distinct preferences for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 tau/theta is primarily expressed in T-cells although it occurs at lower levels in the brain, heart, pancreas placenta and kidneys. It plays a role in cell cycle progression via interaction with p27(Kip1). Along with COPI, the theta isoform binds the GB1 RSR sequence involved in protein trafficking. The isoform forms a complex with Hsp60 and cellular prion protein which may be involved in prion diseases. Elevated levels of 14-3-3 tau/theta are observed in amyotrophic lateral sclerosis. 14-3-3 tau/theta also binds BCR/Abl, bacterial effector protein Tir, yes-associated protein and the FSH receptor.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: p-YWHAQ (S232) polyclonal antibody detects endogenous levels of YWHAQ protein only when phosphorylated at Ser232.
W4BiowMW: ~ 28 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human YWHAQ around the phosphorylation site of Serine 232.Alternative Name:
14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1; YWHAQWestern blot analysis: Western blot (WB) analysis of p-YWHAQ (S232) polyclonal antibody at 1:500 dillution Lane1: Hela whole cell lysate Lane2: Hela treated with LPS(100ng/ml, 30min) whole cell lysate Lane3: C6 whole cell lysate Lane4: C6 treated with EGF(0.1ng/ml, 30min) whole cell lysate Lane5: BV2 whole cell lysate Lane6: BV2 treated with LPS(100ng/ml, 30min) whole cell lysate
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P27348
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