RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
CD2 Antibodies
Mouse anti-human CD2 antibodies
-3 clones CLB-T11/1,6G4 (purified & FITC)
XIX.8 (purified, FITC & PE)
GJ12 (purified, FITC ,PE, & R-PE/CY5 )
-see specialty T cells stimulation clones
mouse monoclonal CD2
CAT# RDI-M1314clb PRICE: $375.00/vial
Code: CLB-CD2 (CLB-T11)
Lot number:
Tests/vial: 200
Specificity:Cell Type Human T lymphocytes Af MW. 50 kD.
Reactivity:The monoclonal antibody is directed against the CD2-antigen (T11-antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with all human peripheral T lymphocytes and 90% of the thymocytes. The monoclonal antibody blocks the rosette-formation of human T lymphocytes with sheep erythrocytes.
Applications:Monitoring of T-cell numbers in peripheral blood. Characterization of leukaemias and lymphomas. Identification of T-cells in tissue. Analysis of NK-cell subset.
Methods: Indirect immunofluorescence staining with analysis by FACS or fluorescence microscopy.
Clone: CLB-163 This clone has been derived from hybridization of SP2/0 cells with spleen cells of a (BALB/c x A/J) mouse immunized with cells of a patient with Sezary Syndrome.
Mouse isotype: The monoclonal antibody is of the mouse subclass IgG1.
Source: Ascites fluid of tumor bearing BALB/c mice.
Purification: Ammoniumsulphate precipitation and ion exchange chromatography.
Packing: Each vial contains 1ml with approximately 0.1mg/ml monoclonal antibody and 10mg BSA in 20 mM TRIS, 150mM NaCI, pH 8.0.
Preservative: Merthiolate (0.001%).
Storage: Monoclonal antibodies should be stored below -18`C. Repeated thawing and freezing may reduce the antibody activity and should be avoided.
IN VITRO RESEARCH USE ONLY
mouse monoclonal CD2,conjugated to Fluorescein
Code: CLB-CD2-F (CLB-T11)
CAT# RDI-M1465clb PRICE: $375.00/vial
Tests/vial: 100
Specificity: Cell type: Human T lymphocytes
Ag.MW.: 50 kD.
Reactivity: The monoclonal antibody is directed against the CD-2-antigen (T11-antigen), which is expressed on human Tlymphocytes. The monoclonal antibody reacts with all human peripheral T lymphocytes and 90% of the thymocyte. The monoclonal antibody blocks the rosette-formation of human T lymphocytes with sheep erythrocytes.
Methods: Direct immunofluorescence staining with analysis by FACS or fluorescence microscopy.
Clone: CLB-163This clone has been derived from hybridization of SP2/0 cells with spleen cells of a (BALB/c x A/J) mouse immunized with cells of a patient with the Sezary syndrome.
Mouse isotype: The monoclonal antibody is of the mouse subclass IgG1.
Source: Ascites fluid of tumour bearing BALB/c mice.
Purification: Ammoniumsulphate precipitation and ion exchange chromatography.
Chemical Characteristics: Molecular F/P ratio : 6.8 Weight F/P ratio : 16.5 x 10-3 Fluorescein concentration : 8.3 x 10-4 mg/ml Optical density ratio (E495/E280) at pH 7.2 : 1.27
Packing: Each vial contains 1 ml with approximately 50 ug/ml conjugated monoclonal antibody and 10 mg BSA in PBS.
Preservative: Merthiolate (0.001%).
Storage: Fluorescein labelled monoclonal antibodies should be stored 4 DEG C.Repeated thawing and freezing may reduce the antibody activity and should be avoided.
FOR IN VITRO RESEARCH USE ONLY
Mouse ANTI-HUMAN CD2+ T LYMPHOCYTES
PRODUCT CODE :RDI-CBL140 $375.00/vial
Also available: FITC labeled cat#RDI-CBL140FT $395.00/100 Tests
PE labeled cat#RDI-CBL140PE $469.00/100 Tests
CLONE: XIX.8
ISOTYPE: IgG2a
SOURCE: Mouse ascitic fluid
PURIFICATION Ion exchange chromatography following ammonium sulphate precipitation.
SPECIFICITY: 50Kd MW lymphocyte surface antigen identified by monoclonal antibodies belonging to the CD2 cluster. The antigen is expressed on all peripheral blood T lymphocytes, majority of thymocytes and malignant cells of T cell origin, including T- ALL cells. Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen. Common ALL cells do not express CD2 antigen.
The antigen identified by CD2 type monoclonal antibodies has been characterised as the receptor for sheep erythrocytes and pre-treatment of lymphocytes with CD2 monoclonal antibodies inhibits E rosette formation. The antigen also functions as the receptor for the CD 58 antigen (LFA-3).
Antigen distribution: T-cells (E+) 100%
Thymocytes 95 � 3%
Peripheral blood lymphocytes 82 � 5%
NK-cells (CD16+) 50 � 10%
Granulocytes < 1%
Monocytes < 1%
B-cells (E-) < 1%
APPLICATIONS: * Identification of T lymphocytes by indirect immunofluorescence staining
* Leukaemia and lymphoma phenotyping
* Identification of normal and malignant T lymphocytes in cryostat sections of frozen tissue
* Immunoprecipitation of the CD2 antigen
* Isolation or removal of T lymphocytes by FACS or panning techniques
REFERENCES: Leucocyte Typing III, ed. McMichael, A.J. et al . Oxford University Press (1987)
PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200�g/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 30 days. Dilute working solutions are best prepared on the day of use.
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
For BULK (without BSA and without Preservative) use cat#RDI-CBL140-1XP $875.00/1 milligram Larger Sizes quoted on Request
Mouse ANTI-HUMAN CD2 ANTIGEN
PRODUCT CODE :RDI-CBL487 $375.00/vial 200ug
Also available: FITC labeled cat#RDI-CBL487FT $375.00/100 Test vial
PE labeled cat#RDI-CBL487PE $469.00/100T vial
R-PE/CY5 cat#RDI-CBL487PC5 $469.00/vial
CLONE GJ12
ISOTYPE IgG1
SOURCE Tissue culture supernatant
PURIFICATION METHOD Protein A affinity chromatography
SPECIFICITY : The antibody reacts with a 50 kDa lymphocyte surface molecule identified by monoclonal antibodies belonging to the CD2 cluster. The antigen is also known as sheep erythrocyte receptor and functions as a receptor for the CD58 antigen (LFA-3) through its NH2 terminal domain. The antigen is expressed on all peripheral blood T lymphocytes, the majority of thymocytes and malignant cells of T cell origin, including T ALL cells. Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen. Common ALL cells do not express CD2 antigen.
Antigen distribution: T cells (E+) 100%
Thymocytes 95 + 3%
Peripheral blood lymphocytes 82 + 5%
NK cells 50 + 10%
B cells (E-) < 1%
Granulocytes < 1%
Monocytes < 1%
APPLICATIONS
*Identification of T lymphocytes by indirect immunofluorescence staining
*Leukaemia and lymphoma phenotyping
*Identification of normal and malignant T lymphocytes on frozen sections
*Immunoprecipitation of the CD2 antigen
REFERENCES Beyers, A. D. et al. Immunol Rev. III, 59-77 (1989)
Bierer, B. E. & Burakoff, S. J. Immunol Rev. III, 267-294 (1989)
PRESENTATION The monoclonal is presented at a concentration of 200�g/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.
For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures